Mechanism of CD11b down-regulation from phorbol myristate acetate stimulated polymorphonuclear neutrophils

Authors

• Khalil A. Aziz, Regional Department of Immunology, Birmingham Heartlands Hospital, Bordesley Green East, Birmingham B9 5SS, United Kingdom. Tel. +44 (121) 4240185. Fax. +44 (121) 4243129. E-mail: khalilazizh{at}yahoo.co.uk

Abstract

OBJECTIVE: To investigate the mechanism of CD11b down-regulation in phorbol myristate acetate (PMA) stimulated polymorphonuclear leukocytes (PMN). METHODS: Purified PMN were stimulated with PMA in the presence, or absence, of various enzyme inhibitors. Following stimulation, PMN CD11b expression was examined by flow cytometry and Western-blotting. The entire work was carried out at Liverpool University between the period of 1998 and 2001. RESULTS: Stimulation of PMN with PMA induced the down-regulation of CD11b by a mechanism involving a combination of an oxidant and a serine protease; most likely the primary granule derived elastase. CONCLUSION: The present study shows for the first time the cooperation of both oxidants and enzymes in the down-regulation of PMN adhesion receptors.

Article Type

Research Article

First Page

1596

Last Page

1601

Recommended Citation

Aziz, Khalil A. (2005) "Mechanism of CD11b down-regulation from phorbol myristate acetate stimulated polymorphonuclear neutrophils," Saudi Medical Journal: Vol. 26: Iss. 10, Article 18.
DOI: https://doi.org/10.15537/1658-3175.3159