Determination of human papillomavirus type 16 genotype and construction of cloning vector pTZ57R encoding HPV16 E7 gene.

Authors

• Zahra Meshkat
• Hoorieh Soleimanjahi
• Mahmoud Mahmoudi
• Hessam Mirshahabi
• Zuhair M. Hassan
• Saeed R. Ghaffari
• Tayebeh Sabokbar

Abstract

OBJECTIVES: To isolate and construct a cloning vector containing the human papillomavirus (HPV)16-E7 gene as a target for application as a DNA vaccine. METHODS: The study was performed in 2005 in Iran. The E7 gene, one of the most important HPV oncoproteins and a target molecule for therapeutic vaccines, was amplified by polymerase chain reaction (PCR). The PCR product was cloned into a suitable cloning vector and confirmed by colony-PCR, restriction enzyme analysis, and sequenced. RESULTS: The desired plasmid was sequenced and indicated 99% homology with those mentioned in the Genbank. CONCLUSIONS: The Iranian HPV16 E7 gene sequence is very similar to other sequences in the Genbank, and it can be used as a candidate gene in a therapeutic vaccine for Iranian patients with cervical cancer.

Article Type

Research Article

First Page

1511

Last Page

1515

Recommended Citation

Meshkat, Zahra; Soleimanjahi, Hoorieh; Mahmoudi, Mahmoud; Mirshahabi, Hessam; Hassan, Zuhair M.; Ghaffari, Saeed R.; and Sabokbar, Tayebeh (2007) "Determination of human papillomavirus type 16 genotype and construction of cloning vector pTZ57R encoding HPV16 E7 gene.," Saudi Medical Journal: Vol. 28: Iss. 10, Article 8.
DOI: https://doi.org/10.15537/1658-3175.4086