Expression of CXC chemokine IP-10/Mob-1 by primary hepatocytes following heat shock

Authors

• Gholamhossein Hassanshahi, Department of Hematology and Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Tel. +98 (391) 5234001-3. Fax. +98 (391) 5225209. E-mail: ghassanshahi{at}gmail.com, ghassanshahi{at}yahoo.co.uk
• Sanjai S. Patel, Department of Hematology and Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Tel. +98 (391) 5234001-3. Fax. +98 (391) 5225209. E-mail: ghassanshahi{at}gmail.com, ghassanshahi{at}yahoo.co.uk
• Abdollah A. Jafarzadeh, Department of Hematology and Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Tel. +98 (391) 5234001-3. Fax. +98 (391) 5225209. E-mail: ghassanshahi{at}gmail.com, ghassanshahi{at}yahoo.co.uk
• Alan J. Dickson, Department of Hematology and Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Tel. +98 (391) 5234001-3. Fax. +98 (391) 5225209. E-mail: ghassanshahi{at}gmail.com, ghassanshahi{at}yahoo.co.uk

Abstract

OBJECTIVE: To examine the expression of chemokine IP-10/Mob-1 of hepatocytes in responses to the stress imposed during isolation by collagenase perfusion. METHODS: This study was performed in the Faculty of Life Sciences University of Manchester during 2001-2005. We employed western and northern blotting analysis to detect IP-10/Mob-1 in isolated and cultured hepatocytes in response to isolation stresses and under heat shock stimulation in this project. RESULTS: We showed that the ELR-CXC chemokine, IP-10/Mob-1 is secreted from isolated rat hepatocytes immediately after isolation and early during culture and IP-10/Mob-1, expression by hepatocytes was also stimulated in response to heat shock. CONCLUSION: It seems that hepatocytes mimic the experiences of liver injury in vivo such as during stress, trauma, or after insults, and therefore, produce stress related agents like IP-10/Mob-1 chemokine to overcome such a injurious condition following isolation and heat shock stimulation. This study also provides a useful model to study the regulation of expression of this chemokines in vitro.

Article Type

Research Article

First Page

514

Last Page

518

Recommended Citation

Hassanshahi, Gholamhossein; Patel, Sanjai S.; Jafarzadeh, Abdollah A.; and Dickson, Alan J. (2007) "Expression of CXC chemokine IP-10/Mob-1 by primary hepatocytes following heat shock," Saudi Medical Journal: Vol. 28: Iss. 4, Article 3.
DOI: https://doi.org/10.15537/1658-3175.3848