A close link between Fas, p53 and Apaf-1 in chronic myeloid leukemia

Authors

• Faris Q. Alenzi, Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, PO Box 2114, Dammam 31451, Kingdom of Saudi Arabia. Tel. +966 (3) 8577000. Fax. +966 (3) 8572872. Email: falenzi{at}kfu.edu.sa
• Richard K. Wyse, Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, PO Box 2114, Dammam 31451, Kingdom of Saudi Arabia. Tel. +966 (3) 8577000. Fax. +966 (3) 8572872. Email: falenzi{at}kfu.edu.sa
• Waleed G. Tamimi, Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, PO Box 2114, Dammam 31451, Kingdom of Saudi Arabia. Tel. +966 (3) 8577000. Fax. +966 (3) 8572872. Email: falenzi{at}kfu.edu.sa
• Mohammad S. Bamaga, Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, PO Box 2114, Dammam 31451, Kingdom of Saudi Arabia. Tel. +966 (3) 8577000. Fax. +966 (3) 8572872. Email: falenzi{at}kfu.edu.sa
• Mahmoud Lotfy, Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, PO Box 2114, Dammam 31451, Kingdom of Saudi Arabia. Tel. +966 (3) 8577000. Fax. +966 (3) 8572872. Email: falenzi{at}kfu.edu.sa

Abstract

In chronic myeloid leukemia CML proliferation is increased and resistance to apoptosis has been proposed as a mechanism accounting for myeloid cell expansion. There is still controversy on whether apoptosis plays an important role in the regulation of myelopoiesis. This study aims to investigate whether apoptosis-related proteins play a role in the evolution of CML and to identify, the relationship between Fas, p53 and apoptosis protease activating factor Apaf-1 in CML. We found increased p53 and Apaf-1 messenger ribonucleic acid mRNA in patients with CML. However, one patient, who had a p53 point mutation, showed a massive elevation of p53 mRNA during blast crisis yet, conversely, a considerable reduction in Apaf-1 mRNA and Fas mRNA. Our results show an in-vivo linkage between Fas, p53 and Apaf-1 transcription regulation. This suggests that key genes involved in apoptosis are also involved in CML disease progression.

Article Type

Case Report

First Page

1119

Last Page

1121

Recommended Citation

Alenzi, Faris Q.; Wyse, Richard K.; Tamimi, Waleed G.; Bamaga, Mohammad S.; and Lotfy, Mahmoud (2007) "A close link between Fas, p53 and Apaf-1 in chronic myeloid leukemia," Saudi Medical Journal: Vol. 28: Iss. 7, Article 25.
DOI: https://doi.org/10.15537/1658-3175.3989