Microarray analysis of micro-ribonucleic acid expression in primary immunoglobulin A nephropathy

Authors

• Yong Dai, Clinical Medical Research Center, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China.
• Weiguo Sui, Clinical Medical Research Center, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China.
• Huijuan Lan, Clinical Medical Research Center, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China.
• Qiang Yan, Clinical Medical Research Center, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China.
• He Huang, Clinical Medical Research Center, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China.
• Yuanshuai Huang, Clinical Medical Research Center, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China.

Abstract

OBJECTIVE: To explore the relationship between immunoglobulin A nephropathy (IgAN) and microRNA (miRNA). METHODS: We analyzed the miRNA expression profiles in renal biopsies from 11 IgAN patients and 3 controls at the Kidney Transplantation and Hemo Purification Center of 181 Hospital, China, from May to October 2007, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. RESULTS: This study identified 132 miRNAs in renal samples, of which 35 miRNAs up-regulated in IgAN biopsies. The chip results were confirmed by northern blot analysis and by quantitative real-time polymerase chain reaction (RT-PCR) tests. CONCLUSION: Our study may help clarify the molecular mechanisms involved in the pathogenesis of IgAN, and miRNAs potentially serve as a novel diagnostic biomarker of IgAN.

Article Type

Research Article

First Page

1388

Last Page

1393

Recommended Citation

Dai, Yong; Sui, Weiguo; Lan, Huijuan; Yan, Qiang; Huang, He; and Huang, Yuanshuai (2008) "Microarray analysis of micro-ribonucleic acid expression in primary immunoglobulin A nephropathy," Saudi Medical Journal: Vol. 29: Iss. 10, Article 2.
DOI: https://doi.org/10.15537/1658-3175.4491