Effects of psychotropic drugs on the thrombin-induced liberation of arachidonate in human platelets

Authors

• Ramadhan Oruch, Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. Tel. +47 (55) 586840. Tel/Fax. +47 (55) 586360. E-mail: ramadhan.oruch{at}biomed.uib.no
• Ian F. Pryme, Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. Tel. +47 (55) 586840. Tel/Fax. +47 (55) 586360. E-mail: ramadhan.oruch{at}biomed.uib.no
• Holm Holmsen, Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. Tel. +47 (55) 586840. Tel/Fax. +47 (55) 586360. E-mail: ramadhan.oruch{at}biomed.uib.no

Abstract

OBJECTIVE: To compare the effects of chlorpromazine (CPZ), prochlorperazine (PCP), trifluoperazine (TFP), clozapine (CLO), haloperidol (HPD), quetiapine (QTP), pimozide (PMZ), and olanzapine (OLP) as well as the tricyclic antidepressants amitriptyline AMI, imipramine IMI, and nortriptyline NTP on thrombin-induced liberation of arachidonic acid AA in platelets. METHODS: This work was carried out at the Department of Biomedicine, University of Bergen, Norway in 2006-2007. Human platelets pre labelled with [3H] arachidonate were incubated with thrombin in the absence and presence of the drugs, and the amount of free [3H] arachidonate liberated was determined. Myosin light chain (MLC) phosphorylation was determined in [32P] phosphate-labelled platelets after sodium dodecyl sulfate polyacrylamide gel electrophoresis. The effects of the drugs on the molecular area and surface pressure of phospholipid monolayers were determined in the Langmuir apparatus. RESULTS: All drugs reduced arachidonate liberation with the ranking order of increasing potency: OLP

Article Type

Research Article

First Page

1397

Last Page

1407

Recommended Citation

Oruch, Ramadhan; Pryme, Ian F.; and Holmsen, Holm (2008) "Effects of psychotropic drugs on the thrombin-induced liberation of arachidonate in human platelets," Saudi Medical Journal: Vol. 29: Iss. 10, Article 4.
DOI: https://doi.org/10.15537/1658-3175.4493