Variation of M3 muscarinic receptor expression in different prostate tissues and its significance

Authors

• Wei Song, Department of Minimally Invasive Urology Center, Shandong Provincial Hospital, The Second Hospital of Shandong University, Jinan, China.
• Mingzhen Yuan, Department of Minimally Invasive Urology Center, Shandong Provincial Hospital, The Second Hospital of Shandong University, Jinan, China.
• Shengtian Zhao, Department of Minimally Invasive Urology Center, Shandong Provincial Hospital, The Second Hospital of Shandong University, Jinan, China.

Abstract

OBJECTIVE: To detect the expression of the muscarinic receptor (M receptor) in different prostate tissues and analyze the role of its subtype in prostatic oncogenesis. METHODS: Thirty-six cases of normal prostate and benign prostatic hyperplasia, and 8 cases of prostatic tumor, were used in this study from the Shandong University, Shandong, China, between 2003-2006. The protein expressions of M1, M2, and M3 receptors in each group were determined by Western-blotting. The gene expressions of the M3 receptor and vascular endothelial growth factors (VEGF) in each group were determined by reverse transcriptase-polymerase chain reaction. RESULTS: The protein and gene expressions of the M3 receptor in the prostatic carcinoma group were higher than that of benign prostatic hyperplasia group (p=0.0001) and normal prostate group (p=0.0001). The M3 receptor and VEGF showed positive straight-line correlations of gene expressions with the 3 groups (r=0.4999, p=0.0001). CONCLUSION: The M3 receptor may have a close relationship with prostatic oncogenesis.

Article Type

Research Article

First Page

1010

Last Page

1016

Recommended Citation

Song, Wei; Yuan, Mingzhen; and Zhao, Shengtian (2009) "Variation of M3 muscarinic receptor expression in different prostate tissues and its significance," Saudi Medical Journal: Vol. 30: Iss. 8, Article 5.
DOI: https://doi.org/10.15537/1658-3175.4794