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Abstract

OBJECTIVE: To determine the relationship between resistin gene RETN C-180G variant and circulating resistin concentration in Saudi colon cancer patients. METHODS: This case-control study was conducted in the Biochemistry Department, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia from April 2009 to December 2009. The serum concentration had been measured with enzyme-linked immunosorbent assay in 60 colon cancer patients and in 60 controls matched in gender and age. The single nucleotide polymorphism SNP C-180G was genotyped using polymerase chain reaction PCR and restriction fragment length polymorphism RFLP techniques. RESULTS: We observed a significantly higher serum resistin level in colon cancer group compared with control group 19.44 ± 8.46 versus 5.45 ± 2.73 ng/ml; p=0.0001, with significant p=0.03 higher levels showed in women than in men in patients and controls. In patients, the heterozygous CG and homozygous GG genotype carriers showed higher p=0.08 levels of serum resistin compared to CC homozygous. This difference was not observed p=0.78 among SNP C-180G genotypes in control group. CONCLUSION: Our result showed no association between the C-180G SNP and the serum resistin concentrations and suggests that the high resistin level in colon cancer patients may play an important role in colon cancer development.

Article Type

Research Article

First Page

495

Last Page

500

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