Association of XRCC1 Trp194 allele with risk of breast cancer, and Ki67 protein status in breast tumor tissues

Authors

• Chiya Jalali, From the Cellular and Molecular Research Center (Jalali, Amini, Abdi), the Department of Internal Medicine (Ghaderi), the Department of Clinical Biochemistry (Abdi), the Department of Epidemiology and Biostatistics (Roshani), Faculty of Medicine, the Liver and Digestive Research Center (Ghaderi), Kurdistan University of Medical Sciences, Sanandaj, Iran
• Bayazid Ghaderi, From the Cellular and Molecular Research Center (Jalali, Amini, Abdi), the Department of Internal Medicine (Ghaderi), the Department of Clinical Biochemistry (Abdi), the Department of Epidemiology and Biostatistics (Roshani), Faculty of Medicine, the Liver and Digestive Research Center (Ghaderi), Kurdistan University of Medical Sciences, Sanandaj, Iran
• Sabrieh Amini, From the Cellular and Molecular Research Center (Jalali, Amini, Abdi), the Department of Internal Medicine (Ghaderi), the Department of Clinical Biochemistry (Abdi), the Department of Epidemiology and Biostatistics (Roshani), Faculty of Medicine, the Liver and Digestive Research Center (Ghaderi), Kurdistan University of Medical Sciences, Sanandaj, Iran
• Mohammad Abdi, From the Cellular and Molecular Research Center (Jalali, Amini, Abdi), the Department of Internal Medicine (Ghaderi), the Department of Clinical Biochemistry (Abdi), the Department of Epidemiology and Biostatistics (Roshani), Faculty of Medicine, the Liver and Digestive Research Center (Ghaderi), Kurdistan University of Medical Sciences, Sanandaj, Iran
• Daem Roshani, From the Cellular and Molecular Research Center (Jalali, Amini, Abdi), the Department of Internal Medicine (Ghaderi), the Department of Clinical Biochemistry (Abdi), the Department of Epidemiology and Biostatistics (Roshani), Faculty of Medicine, the Liver and Digestive Research Center (Ghaderi), Kurdistan University of Medical Sciences, Sanandaj, Iran

Abstract

Objectives: To evaluate the role of this polymorphism as a risk factor for breast cancer in Kurdish patients and to investigate the possible association between Arg194Trp x-ray repair cross-complementing group 1 (XRCC1) gene polymorphisms with clinical and histopathological outcomes of patients with breast cancer. Methods: A total of 100 breast cancer patients and 200 cancer-free controls in Kurdish population of Kurdistan state admitted to Tohid Hospital, Sanandaj, Kurdistan, Iran between January 2012 and May 2015 were enrolled in this cross-sectional study. Tissue expression of estrogen receptor (ER), progesteron receptor (PR), human epidermal growth factor receptor 2 (Her2/neu), and Ki67 were evaluated by immunohistochemistry (IHC). The Arg194Trp genotypes were determined by polymerase chain reaction- restriction fragment length polymorphism method. Results: Our data showed that the risk for breast cancer increased significantly among the Trp variant of XRCC1. Statistically significant association was found between codon 194 polymorphisms and tissue expression of Ki67. Conclusion: The Trp allele of codon 194 XRCC1 is a potential risk factor for breast cancer in Kurdish ethnicity. Furthermore, effect of this polymorphism on clinical and histological features of breast cancer was significant.

Article Type

Research Article

First Page

624

Last Page

630

Recommended Citation

Jalali, Chiya; Ghaderi, Bayazid; Amini, Sabrieh; Abdi, Mohammad; and Roshani, Daem (2016) "Association of XRCC1 Trp194 allele with risk of breast cancer, and Ki67 protein status in breast tumor tissues," Saudi Medical Journal: Vol. 37: Iss. 6, Article 4.
DOI: https://doi.org/10.15537/smj.2016.6.13540