First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals

Authors

• Reem Y. Aljindan, From the Department of Microbiology (Aljindan), College of Medicine, from the Department of Clinical Laboratory Science (Hussein), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University; from the Department of Pathology and Laboratory Medicine (Khoudair, Shaikh, Alabdulqader, Abdalhamid), King Fahad Specialist Hospital, Dammam, Kingdom of Saudi Arabia; Department of Microbiology (Hassan), College of Medicine, Alexandria University, Alexandria, Egypt; and from the Department of Medicine (Shorman), University of Tennessee, Knoxville, TN, United States of America
• Nasreldin E. Hussein, From the Department of Microbiology (Aljindan), College of Medicine, from the Department of Clinical Laboratory Science (Hussein), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University; from the Department of Pathology and Laboratory Medicine (Khoudair, Shaikh, Alabdulqader, Abdalhamid), King Fahad Specialist Hospital, Dammam, Kingdom of Saudi Arabia; Department of Microbiology (Hassan), College of Medicine, Alexandria University, Alexandria, Egypt; and from the Department of Medicine (Shorman), University of Tennessee, Knoxville, TN, United States of America
• Hala A. Khoudair, From the Department of Microbiology (Aljindan), College of Medicine, from the Department of Clinical Laboratory Science (Hussein), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University; from the Department of Pathology and Laboratory Medicine (Khoudair, Shaikh, Alabdulqader, Abdalhamid), King Fahad Specialist Hospital, Dammam, Kingdom of Saudi Arabia; Department of Microbiology (Hassan), College of Medicine, Alexandria University, Alexandria, Egypt; and from the Department of Medicine (Shorman), University of Tennessee, Knoxville, TN, United States of America
• Alaa Y. Shaikh, From the Department of Microbiology (Aljindan), College of Medicine, from the Department of Clinical Laboratory Science (Hussein), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University; from the Department of Pathology and Laboratory Medicine (Khoudair, Shaikh, Alabdulqader, Abdalhamid), King Fahad Specialist Hospital, Dammam, Kingdom of Saudi Arabia; Department of Microbiology (Hassan), College of Medicine, Alexandria University, Alexandria, Egypt; and from the Department of Medicine (Shorman), University of Tennessee, Knoxville, TN, United States of America
• Hoda A. Hassan, From the Department of Microbiology (Aljindan), College of Medicine, from the Department of Clinical Laboratory Science (Hussein), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University; from the Department of Pathology and Laboratory Medicine (Khoudair, Shaikh, Alabdulqader, Abdalhamid), King Fahad Specialist Hospital, Dammam, Kingdom of Saudi Arabia; Department of Microbiology (Hassan), College of Medicine, Alexandria University, Alexandria, Egypt; and from the Department of Medicine (Shorman), University of Tennessee, Knoxville, TN, United States of America
• Nawar A. Alabdulqader, From the Department of Microbiology (Aljindan), College of Medicine, from the Department of Clinical Laboratory Science (Hussein), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University; from the Department of Pathology and Laboratory Medicine (Khoudair, Shaikh, Alabdulqader, Abdalhamid), King Fahad Specialist Hospital, Dammam, Kingdom of Saudi Arabia; Department of Microbiology (Hassan), College of Medicine, Alexandria University, Alexandria, Egypt; and from the Department of Medicine (Shorman), University of Tennessee, Knoxville, TN, United States of America
• Mahmoud A. Shorman, From the Department of Microbiology (Aljindan), College of Medicine, from the Department of Clinical Laboratory Science (Hussein), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University; from the Department of Pathology and Laboratory Medicine (Khoudair, Shaikh, Alabdulqader, Abdalhamid), King Fahad Specialist Hospital, Dammam, Kingdom of Saudi Arabia; Department of Microbiology (Hassan), College of Medicine, Alexandria University, Alexandria, Egypt; and from the Department of Medicine (Shorman), University of Tennessee, Knoxville, TN, United States of America
• Baha A. Abdalhamid, From the Department of Microbiology (Aljindan), College of Medicine, from the Department of Clinical Laboratory Science (Hussein), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University; from the Department of Pathology and Laboratory Medicine (Khoudair, Shaikh, Alabdulqader, Abdalhamid), King Fahad Specialist Hospital, Dammam, Kingdom of Saudi Arabia; Department of Microbiology (Hassan), College of Medicine, Alexandria University, Alexandria, Egypt; and from the Department of Medicine (Shorman), University of Tennessee, Knoxville, TN, United States of America

Abstract

Objectives: To detect resistance genes to fluoroquinolones and β-lactams in Salmonella strains from a Saudi hospital. Methods: From October 2015 to December 2016, a total of 149 Salmonella strains were collected from stool specimens from patients admitted to King Fahad Hospital of the University, AlKhobar, Saudi Arabia using CHROMagar Salmonella . The organism identification and antimicrobial susceptibility testing were performed using Vitek 2 system. Strain serogrouping was performed using Wellcolex color Salmonella kit. Fluoroquinolone resistance genes, extended-spectrum β-lactamases (ESBLs), and AmpC β-lactamase were determined using polymerase chain reaction (PCR). Enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) was used to determine clonal relatedness. Results: The resistance rates to cefotaxime were 1.3% and ciprofloxacin 19.5%. Plasmid mediated quinolone resistance (PMQR) genes, qnrB and qnrS, were detected in 8 strains, qnrB (n=5) and qnrS (n=3), respectively. No ESBLs, AmpC, or mutations in the topoisomerases were detected. Salmonella isolates formed 7 clusters with similarity. Conclusions: This study reveals the emergence of fluoroquinolone resistant Salmonella in the region imposing public health concerns.

Article Type

Research Article

First Page

685

Last Page

689

Recommended Citation

Aljindan, Reem Y.; Hussein, Nasreldin E.; Khoudair, Hala A.; Shaikh, Alaa Y.; Hassan, Hoda A.; Alabdulqader, Nawar A.; Shorman, Mahmoud A.; and Abdalhamid, Baha A. (2018) "First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals," Saudi Medical Journal: Vol. 39: Iss. 7, Article 7.
DOI: https://doi.org/10.15537/smj.2018.7.22532