Association between preeclampsia and prostasin polymorphism in Pakistani females

Authors

• Saima Ejaz, From the Department of Physiology (Ejaz, Almazyad), King Saud Bin Abdulaziz University of Health Sciences, Riyadh, Kingdom of Saudi Arabia, from the Department of Physiology (Ali), University of Karachi, from the Department of Bioscience (Azim, Bilal), Muhammad Ali Jinnah University, from the Department of Pathology (Khan), Dow University of Health Sciences, Karachi, and from the Department of Physiology (Mahmood), Bhitai Medical and Dental College, Mirpur Khas, Pakistan
• Anwar Ali, From the Department of Physiology (Ejaz, Almazyad), King Saud Bin Abdulaziz University of Health Sciences, Riyadh, Kingdom of Saudi Arabia, from the Department of Physiology (Ali), University of Karachi, from the Department of Bioscience (Azim, Bilal), Muhammad Ali Jinnah University, from the Department of Pathology (Khan), Dow University of Health Sciences, Karachi, and from the Department of Physiology (Mahmood), Bhitai Medical and Dental College, Mirpur Khas, Pakistan
• Kamran Azim, From the Department of Physiology (Ejaz, Almazyad), King Saud Bin Abdulaziz University of Health Sciences, Riyadh, Kingdom of Saudi Arabia, from the Department of Physiology (Ali), University of Karachi, from the Department of Bioscience (Azim, Bilal), Muhammad Ali Jinnah University, from the Department of Pathology (Khan), Dow University of Health Sciences, Karachi, and from the Department of Physiology (Mahmood), Bhitai Medical and Dental College, Mirpur Khas, Pakistan
• Atif Mahmood, From the Department of Physiology (Ejaz, Almazyad), King Saud Bin Abdulaziz University of Health Sciences, Riyadh, Kingdom of Saudi Arabia, from the Department of Physiology (Ali), University of Karachi, from the Department of Bioscience (Azim, Bilal), Muhammad Ali Jinnah University, from the Department of Pathology (Khan), Dow University of Health Sciences, Karachi, and from the Department of Physiology (Mahmood), Bhitai Medical and Dental College, Mirpur Khas, Pakistan
• Asif I. Khan, From the Department of Physiology (Ejaz, Almazyad), King Saud Bin Abdulaziz University of Health Sciences, Riyadh, Kingdom of Saudi Arabia, from the Department of Physiology (Ali), University of Karachi, from the Department of Bioscience (Azim, Bilal), Muhammad Ali Jinnah University, from the Department of Pathology (Khan), Dow University of Health Sciences, Karachi, and from the Department of Physiology (Mahmood), Bhitai Medical and Dental College, Mirpur Khas, Pakistan
• Tuline A. Almazyad, From the Department of Physiology (Ejaz, Almazyad), King Saud Bin Abdulaziz University of Health Sciences, Riyadh, Kingdom of Saudi Arabia, from the Department of Physiology (Ali), University of Karachi, from the Department of Bioscience (Azim, Bilal), Muhammad Ali Jinnah University, from the Department of Pathology (Khan), Dow University of Health Sciences, Karachi, and from the Department of Physiology (Mahmood), Bhitai Medical and Dental College, Mirpur Khas, Pakistan
• Bushra Bilal, From the Department of Physiology (Ejaz, Almazyad), King Saud Bin Abdulaziz University of Health Sciences, Riyadh, Kingdom of Saudi Arabia, from the Department of Physiology (Ali), University of Karachi, from the Department of Bioscience (Azim, Bilal), Muhammad Ali Jinnah University, from the Department of Pathology (Khan), Dow University of Health Sciences, Karachi, and from the Department of Physiology (Mahmood), Bhitai Medical and Dental College, Mirpur Khas, Pakistan

Abstract

Objectives: To investigate the relationship between a prostasin gene variations and the development of preeclampsia in a Pakistani female population. Methods: This was a case-control study carried out at University of Karachi, Karachi, Pakistan between May 2018 and 2019. A single nucleotide polymorphism (SNP) at rs12597511 locus was examined with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses in 76 preeclamptic and 74 normotensive expecting mothers. Results: We observed significantly increased risk of preeclampsia associated with the CC genotype of rs12597511 polymorphism as compared to TT ( p <0.001, OR=8.08, 95% CI:1.28-31.19) and TT/TC ( p <0.001, OR=14.66 and 95% CI: 3.31-65.07) genotypes carriers. Calculation of the allelic distribution revealed a higher frequency of the T allele (82%) among controls; however, the C allele was more prevalent in the preeclamptic group (36%) significantly. Conclusion: The significantly higher C allele frequency in the prostasin gene at the rs12597511 locus in the preeclamptic group indicates that the distribution of the C allele of the prostasin gene is a potential risk factor contributing to the development of preeclampsia.

Article Type

Research Article

First Page

1234

Last Page

1240

Recommended Citation

Ejaz, Saima; Ali, Anwar; Azim, Kamran; Mahmood, Atif; Khan, Asif I.; Almazyad, Tuline A.; and Bilal, Bushra (2020) "Association between preeclampsia and prostasin polymorphism in Pakistani females," Saudi Medical Journal: Vol. 41: Iss. 11, Article 11.
DOI: https://doi.org/10.15537/smj.2020.11.25497