Single nucleotide polymorphisms in cytokine genes and their association with primary Sjögren’s syndrome in Saudi patients: A cross-sectional study

Authors

• Bashaer Alqahtani, From the Department of Zoology (Alqahtani, Daghestani); from the Department of Medicine (Omair), Rheumatology Division; from the Department of Medicine (Alhamad), Pulmonary Division; from the Department of Statistics and Operations Research (Tashkandy), College of Sciences; from the Central Laboratory (Othman); from the Department of Biochemistry (Warsy), King Saud University; from the Department of Clinical Sciences (Alenzi), College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; and from Department of Clinical Sciences (Halwani), Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
• Maha Daghestani, From the Department of Zoology (Alqahtani, Daghestani); from the Department of Medicine (Omair), Rheumatology Division; from the Department of Medicine (Alhamad), Pulmonary Division; from the Department of Statistics and Operations Research (Tashkandy), College of Sciences; from the Central Laboratory (Othman); from the Department of Biochemistry (Warsy), King Saud University; from the Department of Clinical Sciences (Alenzi), College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; and from Department of Clinical Sciences (Halwani), Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
• Mohammed A. Omair, From the Department of Zoology (Alqahtani, Daghestani); from the Department of Medicine (Omair), Rheumatology Division; from the Department of Medicine (Alhamad), Pulmonary Division; from the Department of Statistics and Operations Research (Tashkandy), College of Sciences; from the Central Laboratory (Othman); from the Department of Biochemistry (Warsy), King Saud University; from the Department of Clinical Sciences (Alenzi), College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; and from Department of Clinical Sciences (Halwani), Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
• Fahidah Alenzi, From the Department of Zoology (Alqahtani, Daghestani); from the Department of Medicine (Omair), Rheumatology Division; from the Department of Medicine (Alhamad), Pulmonary Division; from the Department of Statistics and Operations Research (Tashkandy), College of Sciences; from the Central Laboratory (Othman); from the Department of Biochemistry (Warsy), King Saud University; from the Department of Clinical Sciences (Alenzi), College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; and from Department of Clinical Sciences (Halwani), Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
• Esam H. Alhamad, From the Department of Zoology (Alqahtani, Daghestani); from the Department of Medicine (Omair), Rheumatology Division; from the Department of Medicine (Alhamad), Pulmonary Division; from the Department of Statistics and Operations Research (Tashkandy), College of Sciences; from the Central Laboratory (Othman); from the Department of Biochemistry (Warsy), King Saud University; from the Department of Clinical Sciences (Alenzi), College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; and from Department of Clinical Sciences (Halwani), Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
• Yusra Tashkandy, From the Department of Zoology (Alqahtani, Daghestani); from the Department of Medicine (Omair), Rheumatology Division; from the Department of Medicine (Alhamad), Pulmonary Division; from the Department of Statistics and Operations Research (Tashkandy), College of Sciences; from the Central Laboratory (Othman); from the Department of Biochemistry (Warsy), King Saud University; from the Department of Clinical Sciences (Alenzi), College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; and from Department of Clinical Sciences (Halwani), Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
• Nashwa Othman, From the Department of Zoology (Alqahtani, Daghestani); from the Department of Medicine (Omair), Rheumatology Division; from the Department of Medicine (Alhamad), Pulmonary Division; from the Department of Statistics and Operations Research (Tashkandy), College of Sciences; from the Central Laboratory (Othman); from the Department of Biochemistry (Warsy), King Saud University; from the Department of Clinical Sciences (Alenzi), College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; and from Department of Clinical Sciences (Halwani), Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
• Arjumand Warsy, From the Department of Zoology (Alqahtani, Daghestani); from the Department of Medicine (Omair), Rheumatology Division; from the Department of Medicine (Alhamad), Pulmonary Division; from the Department of Statistics and Operations Research (Tashkandy), College of Sciences; from the Central Laboratory (Othman); from the Department of Biochemistry (Warsy), King Saud University; from the Department of Clinical Sciences (Alenzi), College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; and from Department of Clinical Sciences (Halwani), Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
• Rabih Halwani, From the Department of Zoology (Alqahtani, Daghestani); from the Department of Medicine (Omair), Rheumatology Division; from the Department of Medicine (Alhamad), Pulmonary Division; from the Department of Statistics and Operations Research (Tashkandy), College of Sciences; from the Central Laboratory (Othman); from the Department of Biochemistry (Warsy), King Saud University; from the Department of Clinical Sciences (Alenzi), College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; and from Department of Clinical Sciences (Halwani), Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.

Abstract

Objectives: To determine the allelic frequencies and effects of genotypic variations in cytokine gene polymorphisms in a Saudi Arabian population. Methods: This cross-sectional study involved 41 patients with Primary Sjögren’s syndrome (pSS) and 71 healthy controls between October 2018 and May 2019. Single nucleotide polymorphisms genotyping was performed using the SEQUENOM MassARRAY ® System, targeting nine polymorphisms in different cytokine genes. Chi-square tests were used to compare the patients and controls. Results: The interleukin-1 beta (IL-1β) rs1143627 CT (control, 52.7%; patients, 21.2%) and TT + CT ( p = 0.003; p =0.033) genotypes were less frequent in patients with pSS than in healthy controls. The C allele in rs10488631 in the interferon regulatory factor 5 (IRF5) gene and the A allele in rs12583006 in the B-cell activating factor (BAFF) gene were associated with an increased risk of pSS development in the patient group. Conclusion: The CT genotype at −31 (rs1143627) in the IL-1β gene was not associated with a high risk of pSS development in the Saudi population, in contrast to what has been verified in other ethnicities. However, the C allele in rs10488631 in IRF-5 and the A allele in rs12583006 in BAFF were associated.

Article Type

Research Article

First Page

1232

Last Page

1239

Recommended Citation

Alqahtani, Bashaer; Daghestani, Maha; Omair, Mohammed A.; Alenzi, Fahidah; Alhamad, Esam H.; Tashkandy, Yusra; Othman, Nashwa; Warsy, Arjumand; and Halwani, Rabih (2023) "Single nucleotide polymorphisms in cytokine genes and their association with primary Sjögren’s syndrome in Saudi patients: A cross-sectional study," Saudi Medical Journal: Vol. 44: Iss. 12, Article 4.
DOI: https://doi.org/10.15537/smj.2023.44.12.20230490